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GST琼脂糖凝胶4FF|谷胱甘肽琼脂糖凝胶4FF BR 进分 英文名称:Glutathione Sepharose 4FF 其他名称:谷胱甘肽琼脂糖凝胶4FF Ligand:glutathione and 10-carbon linker arm Ligand concentration:120-320umol glutathione/ml medium Binding capacity:~10mg recombinant glutathione S-transferase/ml medium,GST Mr26000 Dynamic binding capacity:~11mg GST-tagged protein/ml medium Mr43000(GSTrap FF 1ml at 1 ml/min=156cm/h) Mean particle size:90um Bead structure:highly cross-linked 4% agarose Maximum flow rate:450cm/h(15ml/min),using xk16/20 column with 5cm bed height,run at room temperature with aqueous buffer Recommended flow rate:Sample loading:<100cm/h(<3ml/min using XK16/20 column);Washing and elution:100~300ml/min using XK16/20 column) PH stability:3-12 Chemical stability:All commonly used aqueous buffers,e.g. 1M acetate pH 4.0 and 6M guanidine hydrochloride for 1 hour at room temperature 性状:乳白色液体 用途:专门用于纯谷胱甘肽S-转移酶(GST)融合蛋白、其他的谷胱甘肽转移酶以及与谷胱甘肽有亲和作用的亲和色谱填料,一步分离就可得到很纯的目标蛋白,纯化条件温和,可保证蛋白的活性 保存:2~8℃
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